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New controlled release systems produced by self-assembly of biopolymers and colloidal particles at fluid-fluid interfaces
Fibril formation of individual pure whey proteins and whey protein isolate (WPI) was studied. The heat-induced conversion of WPI monomers into fibrils at pH 2 and low ionic strength increased with heating time and protein concentration. Previous studies, using a precipitation method, size-exclusion method, or proton NMR spectroscopy, reported a wide range of values for the conversion. An alternative method was developed, namely centrifugal filtration, giving a consistent picture of the conversion. The present results help to explain the disparities reported in literature. No fibrils formed upon heating pure alpha-lactalbumin or pure BSA at pH 2, whereas fibrils formed in pure beta-lactoglobulin (beta-lg) and WPI solutions. Experiments indicate that beta-lg was the only whey protein involved in fibril formation. In all whey protein samples, hydrolysis occurred during heating at pH 2, as determined by HPLC and SDS-PAGE. When WPI fibrils formed at pH 2 were stored at pH 7 or 10, disulphide bonds were formed in the samples. (c) 2006 Elsevier Ltd. All rights reserved. [hide]
Scientific Board
Andreas Bausch
TU Munich, Germany ►
Peter Fischer
ETH Zurich, Switzerland ►
Anne-Marie Hermansson
SIK, Sweden ►
Martin Kroger
ETH Zurich, Germany/Switzerland ►
Erik van der Linden
Wageningen UR, The Netherlands ►
Niklas Loren
SIK, Sweden ►
Leonard Sagis
Wageningen UR, The Netherlands ►
Erich Windhab
ETH Zurich, Switzerland ►
Klaas-Jan Zuidam
Unilever, The Netherlands ►
Scientific Stuff
Manuela Duxenneuner
ETH Zurich, Switzerland ►
Sophia Fransson
SIK, Sweden ►
Nam-Phuong Humblet-Hua
Wageningen UR, The Netherlands ►
Joeska Husny
ETH Zurich, Australia/Switzerland ►
Orit Peleg
ETH Zurich, Israel/Switzerland ►
Cyrille Vezy
TU Munich, Germany ►
Varvara Mitropoulos
ETH Zurich, Switzerland ►
Associated Scientists
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SY Tee, AR Bausch, PA Janmey,
The mechanical cell
CURRENT BIOLOGY 19 (2009) R745 ►Selected conferences (co-)organized by project members
8th World Congress on Computational Mechanics WCCM8 2008
30 June - 5 July 2007, Venice, Italy ►13 May 2025
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